Non-invasive detection of early retinal neuronal degeneration by ultrahigh resolution optical coherence tomography

Tudor, Debbie, Kajic, Vedran, Rey, Sara, Erchova, Irina, Povazay, Boris, Hofer, Bernd, Powell, Kathryn A., Marshall, David, Rosin, Paul L. ORCID: https://orcid.org/0000-0002-4965-3884, Drexler, Wolfgang and Morgan, James E. ORCID: https://orcid.org/0000-0002-8920-1065 2014. Non-invasive detection of early retinal neuronal degeneration by ultrahigh resolution optical coherence tomography. PLoS ONE 9 (4) , e93916. 10.1371/journal.pone.0093916

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Abstract

Optical coherence tomography (OCT) has revolutionises the diagnosis of retinal disease based on the detection of microscopic rather than subcellular changes in retinal anatomy. However, currently the technique is limited to the detection of microscopic rather than subcellular changes in retinal anatomy. However, coherence based imaging is extremely sensitive to both changes in optical contrast and cellular events at the micrometer scale, and can generate subtle changes in the spectral content of the OCT image. Here we test the hypothesis that OCT image speckle (image texture) contains information regarding otherwise unresolvable features such as organelle changes arising in the early stages of neuronal degeneration. Using ultrahigh resolution (UHR) OCT imaging at 800 nm (spectral width 140 nm) we developed a robust method of OCT image analyses, based on spatial wavelet and texture-based parameterisation of the image speckle pattern. For the first time we show that this approach allows the non-invasive detection and quantification of early apoptotic changes in neurons within 30 min of neuronal trauma sufficient to result in apoptosis. We show a positive correlation between immunofluorescent labelling of mitochondria (a potential source of changes in cellular optical contrast) with changes in the texture of the OCT images of cultured neurons. Moreover, similar changes in optical contrast were also seen in the retinal ganglion cell- inner plexiform layer in retinal explants following optic nerve transection. The optical clarity of the explants was maintained throughout in the absence of histologically detectable change. Our data suggest that UHR OCT can be used for the non-invasive quantitative assessment of neuronal health, with a particular application to the assessment of early retinal disease.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Computer Science & Informatics Medicine Optometry and Vision Sciences Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Apoptosis; cell staining; cellular structures and organelles; image analysis; imaging techniques; mitochondria; texture; tomography.
Additional Information:	Pdf uploaded in accordance with the publisher’s policy at http://www.sherpa.ac.uk/romeo/issn/1932-6203/ (accessed 10/07/2014)
Publisher:	Public Library of Science
ISSN:	1932-6203
Funders:	Biotechnology and Biological Sciences Research Council
Date of First Compliant Deposit:	30 March 2016
Last Modified:	11 Oct 2023 21:46
URI:	https://orca.cardiff.ac.uk/id/eprint/61178

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