Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability

Ajduk, Anna, Ilozue, Tagbo, Windsor, Shane, Yu, Yuansong, Seres, K. Bianka, Bomphrey, Richard J., Tom, Brian D., Swann, Karl ORCID: https://orcid.org/0000-0002-4355-1449, Thomas, Adrian, Graham, Chris and Zernicka-Goetz, Magdalena 2011. Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability. Nature Communications 2 , 417. 10.1038/ncomms1424

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Abstract

Fertilization-induced cytoplasmic flows are a conserved feature of eggs in many species. However, until now the importance of cytoplasmic flows for the development of mammalian embryos has been unknown. Here, by combining a rapid imaging of the freshly fertilized mouse egg with advanced image analysis based on particle image velocimetry, we show that fertilization induces rhythmical cytoplasmic movements that coincide with pulsations of the protrusion forming above the sperm head. We find that these movements are caused by contractions of the actomyosin cytoskeleton triggered by Ca2+ oscillations induced by fertilization. Most importantly, the relationship between the movements and the events of egg activation makes it possible to use the movements alone to predict developmental potential of the zygote. In conclusion, this method offers, thus far, the earliest and fastest, non-invasive way to predict the viability of eggs fertilized in vitro and therefore can potentially improve greatly the prospects for IVF treatment.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RG Gynecology and obstetrics
Uncontrolled Keywords:	Biological Sciences; Cell biology; Developmental biology
Publisher:	Nature Publishing Group
ISSN:	2041-1723
Last Modified:	20 Oct 2022 08:51
URI:	https://orca.cardiff.ac.uk/id/eprint/29844

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