Emergence and persistence of integron structures harbouring VIM genes in the Children's Memorial Health Institute, Warsaw, Poland, 1998-2006

Patzer, Jan A., Walsh, Timothy Rutland ORCID: https://orcid.org/0000-0003-4315-4096, Weeks, Janis Lesley, Dzierzanowska, Danuta and Toleman, Mark Alexander Howard ORCID: https://orcid.org/0000-0002-9497-0512 2009. Emergence and persistence of integron structures harbouring VIM genes in the Children's Memorial Health Institute, Warsaw, Poland, 1998-2006. Journal of Antimicrobial Chemotherapy 63 (2) , pp. 269-273. 10.1093/jac/dkn512

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Abstract

Objectives The aim was to perform a genetically detailed study of the emergence of metallo-β-lactamase (MBL) genes in Pseudomonas spp. in the Children's Memorial Health Institute over a 9 year period. Methods Carbapenem-resistant Pseudomonas spp. isolates were collected from 1998 to 2006 and screened for MBL production. MBL-positive isolates were further investigated by a combination of genetic techniques including PCR, genomic location experiments using pulsed-field gel electrophoresis (PFGE) of I-Ceu1, S1 and SpeI digests, and sequencing. Results Of the 20 MBL-containing Pseudomonas isolates collected from 1998 to 2006, 16 Pseudomonas aeruginosa isolates contained an identical class 1 integron structure. Two P. aeruginosa isolates contained the blaVIM-2 gene, and two Pseudomonas putida isolates harboured the blaVIM-4 gene cassette in different integron structures. PFGE analysis indicated that all blaVIM-4-containing P. aeruginosa isolates were closely related, whereas the P. putida isolates were not. All MBL genes in this study were chromosomally encoded, and all isolates harboured only one class 1 integron. The blaVIM-2 isolates were clonal, and the genetic structure supporting the blaVIM-2 gene was found in an identical chromosomal position. Conclusions MBL gene emergence in this hospital has paralleled a 6-fold increase in carbapenem usage. We have found an increase in MBL gene diversity, MBL host organisms and MBL genetic support structures in the hospital over the 9 year study period. There is also compelling evidence of the persistence of individual strains in the hospital throughout the study period. This suggests that once MBL genes have emerged in a hospital environment, they are difficult to remove.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General) R Medicine > RJ Pediatrics > RJ101 Child Health. Child health services
Uncontrolled Keywords:	metallo-β-lactamases, class 1 integrons, VIM-2, VIM-4
Publisher:	Oxford University Press
ISSN:	0305-7453
Last Modified:	20 Oct 2022 07:54
URI:	https://orca.cardiff.ac.uk/id/eprint/26843

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