Root growth inhibition and induction of DNA damage in soybean (Glycine max) by chlorobenzenes in contaminated soil

Liu, Wan, Yang, Yuesuo, Li, P., Zhou, Q. and Sun, T. 2004. Root growth inhibition and induction of DNA damage in soybean (Glycine max) by chlorobenzenes in contaminated soil. Chemosphere 57 (2) , pp. 101-106. 10.1016/j.chemosphere.2004.04.045

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Abstract

The cytotoxic and genotoxic effects of 1,2,4-trichlorobenzene (TCB), chlorobenzene (CB), and hexachlorobenzene (HCB) on root growth and DNA strand breakage damage of soybean nuclei in the test soil were studied using the comet assay. Results indicated that the root growth was significantly inhibited, and DNA strand breaks and the comet tail in the root tip nuclei were both induced after 48 h exposure with TCB concentrations of 50, 100, 200, 300 μg g−1 in the soil. DNA strand breakage was more sensitive to the TCB than the root growth. There was a significant dose–response relationship between the TCB exposure and DNA strand breakage in the soybean nuclei. Thus it is possible for DNA strand breakage to be used as a biomarker of soybean exposed to TCB contamination. Significant cytotoxic threshold concentration of the TCB exposure on the root growth inhibition was determined as 61 μg g−1 in the soil. The toxicity of 100–1000 μg g−1 CB and HCB to the soybean seedlings in the soil were not observed after 48 h or longer exposure.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Earth and Environmental Sciences
Uncontrolled Keywords:	DNA strand breakage ; Comet assay ; Biomarker ; Chlorobenzenes ; Soybean ; Root growth
Publisher:	Elsevier
ISSN:	00456535
Last Modified:	19 Mar 2016 22:03
URI:	https://orca.cardiff.ac.uk/id/eprint/1265

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