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Unbiased molecular analysis of T cell receptor expression using template-switch achored RT-PCR

Quigley, Máire F., Almeida, Jorge R., Price, David and Douek, Daniel C. 2011. Unbiased molecular analysis of T cell receptor expression using template-switch achored RT-PCR. Current Protocols in Immunology , 94:VII:10.33:10.33.1–10.33.16.. 10.1002/0471142735.im1033s94

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Abstract

A detailed knowledge of the principles that guide clonal selection within the memory and effector T cell pools is essential to further our understanding of the factors that influence effective T cell-mediated immunity and has direct implications for the rational design of vaccines and immunotherapies. This unit provides methods for the unbiased quantification and characterization of all expressed T cell receptor (TCR) gene products within any defined T cell population. The approach is based on a template-switch anchored reverse transcription–polymerase chain reaction (RT-PCR) and is optimized for the analysis of antigen-specific T cells isolated directly ex vivo.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Uncontrolled Keywords: T cell receptor;polymerase chain reaction;antigen-specific T cell
Publisher: Wiley-Blackwell
Date of First Compliant Deposit: 16 March 2017
Last Modified: 04 Jun 2017 09:45
URI: http://orca-mwe.cf.ac.uk/id/eprint/99075

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