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An in vitro versus in vivo toxicogenomics investigation of prenatal exposures to tobacco smoke

Perry, Iain, Sexton, Keith, Prytherch, Zoe, Jason, Blum, Judith, Zelikoff and Berube, Kelly 2018. An in vitro versus in vivo toxicogenomics investigation of prenatal exposures to tobacco smoke. Applied In Vitro Toxicology 4 (4) , pp. 379-388. 10.1089/aivt.2016.0041

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Abstract

Approximately 1 million women smoke during pregnancy despite evidence demonstrating serious juvenile and/or adult diseases being linked to early-life exposure to cigarette smoke. Susceptibility could be determined by factors in previous generations, i.e. pre-natal or ‘maternal’ exposures to toxins. Pre-natal exposure to airborne pollutants such as mainstream cigarette smoke has been shown to induce early-life insults (i.e. gene changes) in Offspring that serve as biomarkers for disease later in life. In this investigation, we have evaluated genome-wide changes in the lungs of mouse Dams and their juvenile Offspring exposed pre-natally to mainstream cigarette smoke. An additional lung model was tested alongside the murine model, as a means to find an alternative in vitro, human tissue-based replacement for the use of animals in medical research. Our toxicogenomic and bioinformatic results indicated that in utero exposure altered the genetic patterns of the foetus that could put them at greater risk for developing a range of chronic illnesses in later-life. The genes altered in the in vitro, cell culture model were reflected in the murine model of pre-natal exposure to MCS. The use of alternative in vitro models derived from human medical waste tissues could be viable options to achieve human end-point data and to conduct research that meets the remits for scientists to undertake the 3Rs practises.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: toxicogenomics, pre-natal exposure, mainstream cigarette smoke, in vitro, in vivo
Publisher: Mary Ann Liebert
ISSN: 2332-1512
Date of First Compliant Deposit: 6 March 2017
Date of Acceptance: 1 March 2017
Last Modified: 25 Oct 2019 22:32
URI: http://orca-mwe.cf.ac.uk/id/eprint/98751

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