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Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior

Cao, Ting, Zhang, Fangting, Cai, Liangyuan, Zhou, Yinglin, Buurma, Niklaas J. and Zhang, Xinxiang 2017. Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior. Analyst 2017 (142) , pp. 987-993. 10.1039/C7AN00083A

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Abstract

G-quadruplex sequences exist in eukaryotic organisms and prokaryotes, and the investigation of interactions between G-quadruplexes and small molecule ligands is important for gene therapy, biosensor fabrication, fluorescence imaging and so on. Here, we investigated the behaviour of methylene blue (MB), an electroactive molecule, in the presence of different intramolecular G-quadruplexes by electrochemical method using a miniaturized electrochemical device based on its intrinsic electrochemical property. Although the effects of MB on different intramolecular G-quadruplex structures are not obvious by circular dichroism spectroscopy, distinct differences in binding affinities of MB with different intramolecular G-quadruplexes were fast and easily observed by the electrochemical technique. At the same time, for the human telomerase G-rich sequence (HT), the diffusion current of MB changed sensitively under different ion conditions due to the formation of different conformations of HT, which indicated that our electrochemical method has the potential to study the influence of metal ions on the conformations of the G-quadruplexes with simplicity, rapid response and low cost. From all these, new stacking mechanism and rule were obtained, which were also validated by docking studies and isothermal titration calorimetry (ITC).

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Subjects: Q Science > QD Chemistry
Publisher: Royal Society of Chemistry
ISSN: 0003-2654
Funders: National Natural Science Foundation of China
Date of First Compliant Deposit: 22 February 2017
Date of Acceptance: 14 February 2017
Last Modified: 19 Feb 2018 03:09
URI: http://orca-mwe.cf.ac.uk/id/eprint/98459

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