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Characterisation of large-conductance calcium-activated potassium channels (BK(Ca)) in human NT2-N cells

Chapman, H, Piggot, C, Andrews, P.W. and Wann, Kenneth Taylor 2007. Characterisation of large-conductance calcium-activated potassium channels (BK(Ca)) in human NT2-N cells. Brain Research 1129 , pp. 15-25. 10.1016/j.brainres.2006.10.060

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Abstract

Large-conductance calcium-activated potassium (BKCa) channels were studied in inside-out patches of human NTERA2 neuronal cells (NT2-N). In symmetrical (140 mM) K+ the channel mean conductance was 265 pS, the current reversing at 0 mV. It was selective (PK / PNa = 20:1) and blocked by internal paxilline and TEA. The open probability–voltage relationship for BKCa was fitted with a Boltzmann function, the V½ being 76.3 mV, 33.6 mV and ? 14.1 mV at 0.1 ?M, 3.3 ?M and 10 ?M [Ca2+]i, respectively. The relationship between open probability and [Ca2+]i was fitted by the Hill equation (Hill coefficient 2.7, half maximal activation at 2.0 ?M [Ca2+]i). Open and closed dwell time histograms were fitted by the sum of two and three voltage-dependent exponentials, respectively. Increasing [Ca2+]i produced both an increase in the longer open time constant and a decrease in the longest closed time constant, so increasing mean open time. “Intracellular” ATP evoked a concentration-dependent increase in NT2-N BKCa activity. At + 40 mV half-maximum activation occurred at an [ATP]i of 3 mM (30 nM [Ca2+]i). ADP and GTP were less potent, and AMP-PNP was inactive. This is the first characterisation of a potassium channel in NT2-N cells showing that it is similar to the BKCa channel of other preparations.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Calcium-activated potassium channel ; Patch-clamp ; Paxilline ; ATP ; Nucleotide
ISSN: 0006-8993
Last Modified: 04 Jun 2017 01:37
URI: http://orca-mwe.cf.ac.uk/id/eprint/979

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