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A Modular Synthetic Device to Calibrate Promoters

Gamermann, D., Montagud, A., Aparicio Sanchez, Pablo, Navarro, E., Triana, J., Villatoro, F. R., Urchueguía, J. F. and Fernández de Córdoba, P. 2012. A Modular Synthetic Device to Calibrate Promoters. Journal of Biological Systems 20 (1) , pp. 37-55. 10.1142/S0218339012500015

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Abstract

In this contribution, a design of a synthetic calibration genetic circuit to characterize the relative strength of different sensing promoters is proposed and its specifications and performance are analyzed via an effective mathematical model. Our calibrator device possesses certain novel and useful features like modularity (and thus the possibility of being used in many different biological contexts), simplicity, being based on a single cell, high sensitivity and fast response. To uncover the critical model parameters and the corresponding parameter domain at which the calibrator performance will be optimal, a sensitivity analysis of the model parameters was carried out over a given range of sensing protein concentrations (acting as input). Our analysis suggests that the half saturation constants for repression, sensing and difference in binding cooperativity (Hill coefficients) for repression are the key to the performance of the proposed device. They furthermore are determinant for the sensing speed of the device, showing that it is possible to produce detectable differences in the repression protein concentrations and in turn in the corresponding fluorescence in less than two hours. This analysis paves the way for the design, experimental construction and validation of a new family of functional genetic circuits for the purpose of calibrating promoters.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Subjects: Q Science > QD Chemistry
Uncontrolled Keywords: Synthetic Genetic Circuits; Synthetic Biology; Calibration; Gene Promoter; Effective Modeling of Gene Circuits; Parameter Analysis
Publisher: World Scientific Publishing
ISSN: 0218-3390
Date of Acceptance: 5 January 2012
Last Modified: 04 Jun 2017 09:35
URI: http://orca-mwe.cf.ac.uk/id/eprint/97300

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