Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ CardiffĀ 
WelshClear Cookie - decide language by browser settings

Use of molecular techniques to study microbial diversity in the skin: Chronic wounds reevaluated

Davies, Charlotte E., Wilson, Melanie J., Hill, Katja E., Stephens, Philip, Hill, C. Michael, Harding, Keith Gordon and Thomas, David William 2001. Use of molecular techniques to study microbial diversity in the skin: Chronic wounds reevaluated. Wound Repair and Regeneration 9 (5) , pp. 332-340. 10.1046/j.1524-475x.2001.00332.x

Full text not available from this repository.

Abstract

The skin is colonized by an array of microorganisms which form its natural microflora. Disruption to the normal barrier function of the skin (due to trauma or disease) may result in invasion of the dermis by opportunistic bacteria. To date, these organisms, which may contribute to the chronicity of skin wounds, have been analyzed solely by culture methods. It is increasingly realized that standard culture methods of analysis do not accurately reflect the full diversity of complex microflora. This review discusses the limitations of traditional culture approaches and reviews recent advances in molecular microbiological techniques which facilitate a more comprehensive characterization of the microflora within clinical samples. The currently available technologies and techniques are described, as is their use in clinical practice and their potential for diagnostic screening. Chronic venous ulceration of the lower limbs is an important skin disorder in which the microflora invading the dermal tissues contribute to the observed delayed healing. Using chronic leg ulcers as a working example, we show how strict culture and molecular microbiological techniques may be employed, for the first time in combination, to definitively characterize the invading microbial community of the dermis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Medicine
Publisher: Wiley
ISSN: 1067-1927
Last Modified: 28 Jan 2018 20:12
URI: http://orca-mwe.cf.ac.uk/id/eprint/96555

Citation Data

Cited 97 times in Google Scholar. View in Google Scholar

Cited 75 times in Scopus. View in Scopus. Powered By ScopusĀ® Data

Actions (repository staff only)

Edit Item Edit Item