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Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin

Roussel, Erwan, Sauvadet, A. L., Chaduteau, C., Fouquet, Y., Charlou, J. L., Prieur, D. and Bonavita, M. A. C. 2009. Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin. Environmental Microbiology 11 (9) , pp. 2446-2462. 10.1111/j.1462-2920.2009.01976.x

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Abstract

The distribution of the archaeal communities in deep subseafloor sediments [0–36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average ~3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0–1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured sedimentary Crenarchaeota. However, the anoxic subsurface methane-poor sediments (below 1.5 mbsf) were dominated by less active archaeal communities, such as the Thermoplasmatales, Marine Benthic Group D and other lineages probably involved in the methane cycle (Methanosarcinales, ANME-2 and DSAG/MBG-B). Moreover, the archaeal diversity of some sediment layers was restricted to only one lineage (Uncultured Euryarchaeota, DHVE6, MBG-B, MG-1 and SAGMEG). Sequences forming two clusters within the Thermococcales order were also present in these cold subseafloor sediments, suggesting that these uncultured putative thermophilic archaeal communities might have originated from a different environment. This study shows a transition between surface and subsurface sediment archaeal communities.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Earth and Ocean Sciences
Subjects: Q Science > QR Microbiology
Publisher: Wiley-Blackwell
ISSN: 1462-2912
Last Modified: 19 Mar 2016 22:15
URI: http://orca-mwe.cf.ac.uk/id/eprint/9332

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