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Diversity and distribution of methane-oxidizing microbial communities associated with different faunal assemblages in a giant pockmark of the Gabon continental margin

Cambon-Bonavita, M. A., Nadalig, T., Roussel, Erwan Georges Philippe, Delage, E., Duperron, S., Caprais, J. C., Boetius, A. and Sibuet, M. 2009. Diversity and distribution of methane-oxidizing microbial communities associated with different faunal assemblages in a giant pockmark of the Gabon continental margin. Deep-Sea Research. Part II.Topical studies in oceanography 56 (23) , pp. 2248-2258. 10.1016/j.dsr2.2009.04.007

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Abstract

A giant 800-m-diameter pockmark named REGAB was discovered on the Gabon continental margin actively emitting methane at a water depth of 3200 m. The microbial diversity in sediments from four different assemblages of chemosynthetic organisms, Mytilidae, Vesicomyidae, Sibogliniclae and a bacterial mat, was investigated using comparative 16S rRNA gene sequence analysis. Aggregates of anaerobic methanotrophic archaea (ANME-2) and bacteria of the Desulfosarcina/Desulfococcus cluster were found in all four chemosynthetic habitats. Fluorescence in situ hybridization targeting the ANME-2/Desulfosarcina/Desulfococcus aggregates showed their presence few centimeters (3-5cm) below the surface of sediment. 16S rRNA gene sequences from all known marine ANME groups were detected in the pockmark sediments, as well as from both known bacterial partners. The archaeal diversity was limited to the ANME cluster for all investigated samples. The bacterial diversity included members of the Proteobacteria, Bacilliales, Cytophaga/Flavobacteria, Verrucomicrobia, JS1 and Actinobacteria clusters. Bacterial 16S rRNA gene sequences related to those of known sulphide-oxidizing symbionts were recovered from tissues of several invertebrates including vesicomyid clams and siboglinid tubeworms of REGAB. (C) 2009 Elsevier Ltd. All rights reserved.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Earth and Ocean Sciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: REGAB ; AOM ; Chemosynthetic ecosystems ; Cold seep ; Faunal assemblage ; Microbial phylogeny ; Meditterranean cold seep ; Mosby mud volcano ; Gulf-of-Mexico ; Anaerobic oxidation ; Marine-sediments ; Sequence alignment ; Black-Sea ; Archaea ; Bathymodiolus ; Symbiosis
Additional Information: Cited References Count:53
Publisher: Elsevier
ISSN: 0967-0645
Date of First Compliant Deposit: 30 March 2016
Last Modified: 19 Mar 2016 22:15
URI: http://orca-mwe.cf.ac.uk/id/eprint/9327

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