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Application of Dolichos biflorus in immunoassay detection of kidney collecting duct biomarkers

Sourial, S., Searchfield, L., Schuppe-Koistinen, I., Betton, G. R., Riccardi, Daniela and Price, S. A. 2010. Application of Dolichos biflorus in immunoassay detection of kidney collecting duct biomarkers. Biomarkers 15 (5) , pp. 424-435. 10.3109/1354750X.2010.483526

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Abstract

Currently there are no biomarkers for detecting collecting duct damage in man. Antibodies to several collecting duct-specific antigens exist but sandwich assays have been difficult to establish due to the need for two different antibodies to the same protein. We hypothesized that a collecting duct-specific lectin could be used in combination with a collecting duct-specific antibody to negate the need for two different antibodies. The collecting duct specificity of selected antibodies (NiCa II 13C2, Pap XI 3C7, HuPaP VII 2B11 and aquaporin 2), was verified by immunohistochemistry. Aquaporin 2 and Pap XI 3C7 were used successfully in setting up assays with the lectin Dolichos biflorus, using the Meso Scale Discovery (MSD) platform. Antigen expression was highest in the papillae of rat and human kidney (corresponding to the greatest density of collecting ducts) and was also present in normal urine. We propose that further qualification and validation would lead to an assay for detecting collecting duct damage in man. Read More: http://informahealthcare.com/doi/abs/10.3109/1354750X.2010.483526

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: R Medicine > R Medicine (General)
R Medicine > RB Pathology
R Medicine > RC Internal medicine
Uncontrolled Keywords: Renal papillary necrosis; renal papillary antigen 1; aquaporin 2; Meso Scale Discovery (MSD)
Publisher: Informa
ISSN: 1354-750X
Last Modified: 04 Jun 2017 02:07
URI: http://orca-mwe.cf.ac.uk/id/eprint/9270

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