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Three- and four-dimensional visualization of cell migration using optical coherence tomography

Rey, Sara Maria, Považay, Boris, Hofer, Bernd, Unterhuber, A., Hermann, Boris, Harwood, Adrian John and Drexler, Wolfgang 2009. Three- and four-dimensional visualization of cell migration using optical coherence tomography. Journal of Biophotonics 2 (6-7) , pp. 370-379. 10.1002/jbio.200910027

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Abstract

Conventionally, cell chemotaxis is studied on two-dimensional (2D) transparent surfaces, due to limitations in optical and image data-collection techniques. However, surfaces that more closely mimic the natural environment of cells are often opaque. Optical coherence tomography (OCT) is a noninvasive label-free imaging technique, which offers the potential to visualize moving cells on opaque surfaces and in three dimensions (3D). Here, we demonstrate that OCT is an effective means of time-lapse videomicroscopy of Dictyostelium cells undergoing 3D (2D+time) cell migration on nitrocellulose substrates and 4D (3D+time) chemotaxis within low-density agarose gels. The generated image sequences are compatible with current computer-based image-analysis software for quantification of cell motility. This demonstrates the utility of OCT for cell tracking and analysis of cell chemotaxis in complex environments. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Biosciences
Neuroscience and Mental Health Research Institute (NMHRI)
Subjects: Q Science > QC Physics
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Uncontrolled Keywords: Optical coherence tomography; noninvasive imaging; cell migration; chemotaxis
Publisher: Wiley-Blackwell
ISSN: 1864-063X
Last Modified: 10 Oct 2017 13:12
URI: http://orca-mwe.cf.ac.uk/id/eprint/9260

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