Biofilm formation and changes in bacterial cell surface hydrophobicity during growth in a CAPD model system

Hanlon, Geoffrey W., Denyer, Stephen Paul, Hodges, N. A., Brant, J. A., Lansley, A. B. and Al-Rustamani, W. A. 2004. Biofilm formation and changes in bacterial cell surface hydrophobicity during growth in a CAPD model system. Journal of Pharmacy and Pharmacology 56 (7) , pp. 847-854. 10.1211/0022357023817

Full text not available from this repository.

Abstract

Peritonitis is a frequent complication of continuous ambulatory peritoneal dialysis (CAPD), with patients suffering recurrent attacks. The microorganisms most frequently implicated in the infection are the skin microflora, in particular, the coagulase-negative staphylococci such as Staphylococcus epidermidis. These microorganisms gain access to the peritoneal cavity via the in-dwelling silicone rubber catheter in the abdominal wall and often persist as biofilms on the surface of the catheter. The surface characteristics of S. epidermidis were monitored during growth in a CAPD in-vitro model together with their ability to adhere to silicone rubber substrata. Fresh dialysis fluid exerted an injurious effect on the cells leading to a decrease in cell numbers but during the simulated dialysis period the cells adapted to the applied stresses. Over a 96-h period in the model both a clinical isolate and a skin isolate of S. epidermidis adopted a more hydrophobic phenotype. The data presented here show that the bacteria grown in this in-vivo reflective CAPD model continually adapt to their environment and become more tolerant to the stresses imposed. The adapted cells were seen to colonise silicone rubber substrata.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
ISSN:	00223573
Last Modified:	19 Mar 2016 22:02
URI:	https://orca.cardiff.ac.uk/id/eprint/907

Citation Data

Cited 11 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item