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Fermentation product butane 2,3 diol induces Ca2+ transients in E. coli through activation of lanthanum-sensitive Ca2+ channels

Campbell, Anthony Keith, Naseem, Riffat, Wann, Kenneth Taylor, Holland, I. Barry and Matthews, Stephanie Beatrix 2007. Fermentation product butane 2,3 diol induces Ca2+ transients in E. coli through activation of lanthanum-sensitive Ca2+ channels. Cell Calcium 41 (2) , pp. 97-106. 10.1016/j.ceca.2006.05.007

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Abstract

The results here are the first demonstration of a physiological agonist opening Ca2+ channels in bacteria. Bacteria in the gut ferment glucose and other substrates, producing alcohols, diols, ketones and acids, that play a key role in lactose intolerance, through the activation of Ca2+ and other ion channels in host cells and neighbouring bacteria. Here we show butane 2,3-diol (5–200 mM; half maximum 25 mM) activates Ca2+ transients in E. coli, monitored by aequorin. Ca2+-transient magnitude depended on external Ca2+ (0.1–10 mM). meso-Butane 2,3-diol was approximately twice as potent as 2R,3R (?) and 2S,3S (+) butane 2,3-diol. There were no detectable effects on cytosolic free Ca2+ of butane 1,3-diol, butane 1,4-diol and ethylene glycol. The glycerol fermentation product propane 1,3-diol only induced significant Ca2+ transients in 10 mM external Ca2. Ca2+ butane 2,3-diol Ca2+ transients were due to activation of Ca2+ influx, followed by activation of Ca2+ efflux. The effect of butane 2,3-diol was abolished by La3+, and markedly reduced as a function of growth phase. These results were consistent with butane 2,3-diol activating a novel La3+-sensitive Ca2+ channel. They have important implications for the role of butane 2,3-diol and Ca2+ in bacterial-host cell signalling.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Uncontrolled Keywords: Calcium channels; Diols; Bacteria; Polyhydroxybutyrate; Aequorin; Lactose intolerance; Lanthanum
ISSN: 01434160
Last Modified: 05 Jun 2017 01:38
URI: http://orca-mwe.cf.ac.uk/id/eprint/902

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