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Differential coherent anti-Stokes Raman scattering microscopy with linearly chirped femtosecond laser pulses

Rocha-Mendoza, I., Langbein, Wolfgang Werner, Watson, Peter Duncan and Borri, Paola 2009. Differential coherent anti-Stokes Raman scattering microscopy with linearly chirped femtosecond laser pulses. Optics Letters 34 (15) , pp. 2258-2260. 10.1364/OL.34.002258

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Abstract

We demonstrate a technique for differential coherent anti-Stokes Raman scattering (CARS) microscopy employing linearly chirped femtosecond laser pulses. By replicating the exciting pump-Stokes pulse pairs to create a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference of each pair by glass dispersion, we can adjust the Raman frequency probed by each pair in an intrinsically stable and cost-effective way. The resulting CARS intensities are detected by a single photomultiplier as sum and difference using phase-sensitive frequency filtering. We demonstrate imaging of polymer beads and living cells with suppressed nonresonant CARS background and improved chemical sensitivity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: The Optical Society (OSA)
ISSN: 0146-9592
Last Modified: 03 Jun 2019 19:17
URI: http://orca-mwe.cf.ac.uk/id/eprint/8869

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