Integration-free reprogramming of lamina propria progenitor cells

Howard-Jones, Rachel Anne ORCID: https://orcid.org/0000-0001-7200-3110, Cheung, O. K. Y., Glen, A., Allen, Nicholas Denby ORCID: https://orcid.org/0000-0003-4009-186X and Stephens, Philip ORCID: https://orcid.org/0000-0002-0840-4996 2016. Integration-free reprogramming of lamina propria progenitor cells. Journal of Dental Research 95 (8) , pp. 882-888. 10.1177/0022034516637579

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Abstract

Producing induced pluripotent stem cells (iPSCs) from human tissue for use in personalized medicine strategies or therapeutic testing is at the forefront of medicine. Therefore, identifying a source of cells to reprogram that is easily accessible via a simple noninvasive procedure is of great clinical importance. Reprogramming these cells to iPSCs through nonintegrating methods for genetic manipulation is paramount for regenerative purposes. Here, we demonstrate reprogramming of oral mucosal lamina propria progenitor cells from patients undergoing routine dental treatment. Reprogramming was performed utilizing nonintegrating plasmids containing all 6 pluripotency genes (OCT4, SOX2, KLF4, NANOG, LIN28, and cMYC). Resulting iPSCs lacked genetic integration of the vector genes and had the ability to differentiate down mesoderm, ectoderm, and endoderm lineages, demonstrating pluripotency. In conclusion, oral mucosal lamina propria progenitor cells represent a source of cells that can be obtained with minimal invasion, as they can be taken concurrently with routine treatments. The resulting integration-free iPSCs therefore have great potential for use in personalized medicine strategies.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Dentistry
Publisher:	Sage
ISSN:	0022-0345
Date of First Compliant Deposit:	23 September 2016
Last Modified:	05 May 2023 20:48
URI:	https://orca.cardiff.ac.uk/id/eprint/88243

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