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Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

Ashelford, Kevin E., Eriksson, Maria E., Allen, Christopher M., D'Amore, Rosalinda, Johansson, Mikael, Gould, Peter, Kay, Suzanne, Millar, Andrew J., Hall, Neil and Hall, Anthony 2011. Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis. Genome Biology 12 (3) , R28. 10.1186/gb-2011-12-3-r28

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Abstract

Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QK Botany
Publisher: Genome Biology
ISSN: 1465-6906
Funders: European Commision: Marie Skłodowska-Curie Actions, Swedish Research Council, Swedish Foundation for Strategic Research, Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, Swedish Governmental Agency for Innovation Systems (VINNOVA), University of Liverpool, Biotechnology and Biological Sciences Research Council, Engineering and Physical Sciences Research Council, Royal Society
Date of Acceptance: 23 March 2011
Last Modified: 05 Jun 2017 05:24
URI: http://orca-mwe.cf.ac.uk/id/eprint/86711

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