O'Sullivan, Louise Anne, Webster, Gordon, Fry, John Christopher, Parkes, Ronald John and Weightman, Andrew 2008. Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments. Journal of Microbiological Methods 75 (3) , pp. 579-581. 10.1016/j.mimet.2008.08.006 |
Official URL: http://dx.doi.org/10.1016/j.mimet.2008.08.006
Abstract
Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by ≈23%.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences Earth and Ocean Sciences |
Subjects: | Q Science > QR Microbiology |
Uncontrolled Keywords: | PCR-DGGE; 16S rRNA genes; Prokaryotic diversity; Bacteria Archaea |
Publisher: | Elsevier |
ISSN: | 0167-7012 |
Last Modified: | 02 Aug 2020 16:51 |
URI: | http://orca-mwe.cf.ac.uk/id/eprint/8642 |
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