Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments

O'Sullivan, Louise Anne, Webster, Gordon, Fry, John Christopher, Parkes, Ronald John and Weightman, Andrew 2008. Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments. Journal of Microbiological Methods 75 (3) , pp. 579-581. 10.1016/j.mimet.2008.08.006

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Abstract

Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by ≈23%.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Earth and Ocean Sciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	PCR-DGGE; 16S rRNA genes; Prokaryotic diversity; Bacteria Archaea
Publisher:	Elsevier
ISSN:	0167-7012
Last Modified:	02 Aug 2020 16:51
URI:	http://orca-mwe.cf.ac.uk/id/eprint/8642

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