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Tissue expression and correlation of a panel of urinary biomarkers following Cisplatin-induced kidney injury

Wadey, Rebecca, Pinches, M. G., Jones, H. B., Riccardi, Daniela and Price, S. A. 2014. Tissue expression and correlation of a panel of urinary biomarkers following Cisplatin-induced kidney injury. Toxicologic Pathology 42 (3) , pp. 591-602. 10.1177/0192623313492044

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In recent years, there has been considerable activity to identify urinary biomarkers of nephrotoxicity as noninvasive measurements with greater sensitivity and specificity than traditional biomarkers, such as serum creatinine and blood urea nitrogen. Our study aimed to use cisplatin-treated rats to evaluate the use of immunohistochemistry directed at multiple urinary biomarkers in kidney tissue. Tissue levels were compared to urinary levels of these biomarkers to demonstrate tissue specificity and sensitivity. These techniques could also be used in studies where urine samples are not available, such as retrospective studies in drug safety testing, to demonstrate the potential utility of using these biomarkers in future preclinical or clinical studies. All of the biomarkers investigated showed either an increase (kidney injury molecule [KIM-1], osteopontin [OPN], and, clusterin) or a decrease (alpha-glutathione S-transferase and trefoil factor 3) except beta 2 microglobulin (β2MG) that showed no significant changes 5 days after 1.0 mg/kg or 2.5 mg/kg cisplatin treatment. All of the biomarkers except β2MG showed utility as tissue biomarkers, but KIM-1 and OPN expression correlated closely with urinary biomarker measurements and reflect tissue damage. Future studies are needed to determine the wider application of these two markers for detecting renal toxicity following administration of other nephrotoxicants.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Sage
ISSN: 0192-6233
Last Modified: 22 Feb 2019 13:17

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