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3D-DIP-Chip: a microarray-based method to measure genomic DNA damage

Powell, James Rees, Bennett, Mark Richard, Evans, Katie Ellen, Yu, Shirong, Webster, Richard Michael, Waters, Raymond, Skinner, Nigel and Reed, Simon Huw 2015. 3D-DIP-Chip: a microarray-based method to measure genomic DNA damage. Scientific Reports 5 , 7975. 10.1038/srep07975

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Abstract

Genotoxins cause DNA damage, which can result in genomic instability. The genetic changes induced have far-reaching consequences, often leading to diseases such as cancer. A wide range of genotoxins exists, including radiations and chemicals found naturally in the environment, and in man-made forms created by human activity across a variety of industries. Genomic technologies offer the possibility of unravelling the mechanisms of genotoxicity, including the repair of genetic damage, enhancing our ability to develop, test and safely use existing and novel materials. We have developed 3D-DIP-Chip, a microarray-based method to measure the prevalence of genomic genotoxin-induced DNA damage. We demonstrate the measurement of both physical and chemical induced DNA damage spectra, integrating the analysis of these with the associated changes in histone acetylation induced in the epigenome. We discuss the application of the method in the context of basic and translational sciences.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Nature Publishing Group
ISSN: 2045-2322
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 14 December 2014
Last Modified: 28 Jun 2019 02:40
URI: http://orca-mwe.cf.ac.uk/id/eprint/84171

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