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Antigen targeting reveals splenic CD169+macrophages as promoters of germinal center B-cell responses

Veninga, Henrike, Borg, Ellen G. F., Vreeman, Kyle, Taylor, Philip R., Kalay, Hakan, van Kooyk, Yvette, Kraal, Georg, Martinez-Pomares, Luisa and den Haan, Joke M.M. 2015. Antigen targeting reveals splenic CD169+macrophages as promoters of germinal center B-cell responses. European Journal of Immunology 45 (3) , pp. 747-757. 10.1002/eji.201444983

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Abstract

Ag delivery to specific APCs is an attractive approach in developing strategies for vaccination. CD169+ macrophages in the marginal zone of the spleen represent a suitable target for delivery of Ag because of their strategic location, which is optimal for the capture of blood‐borne Ag and their close proximity to B cells and T cells in the white pulp. Here we show that Ag targeting to CD169+ macrophages in mice resulted in strong, isotype‐switched, high‐affinity Ab production and the preferential induction and long‐term persistence of Ag‐specific GC B cells and follicular Th cells. In agreement with these observations, CD169+ macrophages retained intact Ag, induced cognate activation of B cells, and increased expression of costimulatory molecules upon activation. In addition, macrophages were required for the production of cytokines that promote B‐cell responses. Our results identify CD169+ macrophages as promoters of high‐affinity humoral immune responses and emphasize the value of CD169 as target for Ag delivery to improve vaccine responses.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: Article first published online 14 January 2015
Publisher: Wiley
ISSN: 0014-2980
Date of Acceptance: 3 December 2014
Last Modified: 02 Jun 2020 13:01
URI: http://orca-mwe.cf.ac.uk/id/eprint/79420

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