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Microinjection methods for neutrophils

Laffafian, Iraj, Lewis, Kimberley, Masterman, K. Benjamin and Hallett, Maurice 2014. Microinjection methods for neutrophils. In: Quinn, Mark T. and DeLeo, Frank R. eds. Neutrophil Methods and Protocols, Methods in Molecular Biology, vol. 1124. Humana Press, pp. 181-187. (10.1007/978-1-62703-845-4_11)

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Abstract

The ability to microinject substances into the cytosol of living neutrophils opens the possibility of manipulating the chemistry within the cell and also of monitoring changes using indicators which otherwise cannot be introduced into the cell. However, neutrophils cannot be microinjected by the conventional glass pipette insertion method. Here, we outline two techniques which work well with neutrophils, namely, SLAM (simple lipid-assisted microinjection) and electroinjection.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Humana Press
ISBN: 978-1-62703-845-4
ISSN: 10643745
Last Modified: 24 Dec 2017 20:27
URI: http://orca-mwe.cf.ac.uk/id/eprint/78962

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