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Use of TD-GC–TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa)

Bell, Luke, Spadafora, Natasha D., Muller, Carsten Theodor ORCID: https://orcid.org/0000-0003-0455-7132, Wagstaff, Carol and Rogers, Hilary Joan ORCID: https://orcid.org/0000-0003-3830-5857 2016. Use of TD-GC–TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa). Food Chemistry 194 , pp. 626-636. 10.1016/j.foodchem.2015.08.043

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Abstract

An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD-GC–TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7 days. Relative amounts of VOCs across 3 time points were significantly different – isothiocyanate-containing molecules being abundant on ‘Day 0’. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf life.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: Available online 14 August 2015
Publisher: Elsevier
ISSN: 0308-8146
Funders: BBSRC Case award, EC 7th framework
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 12 August 2015
Last Modified: 05 May 2023 18:18
URI: https://orca.cardiff.ac.uk/id/eprint/76326

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