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Aptamer-MIP hybrid receptor for highly sensitive electrochemical detection of prostate specific antigen

Jolly, Pawan, Tamboli, Vibha, Harniman, Robert L., Estrela, Pedro, Allender, Chris J. and Bowen, Jenna L. 2016. Aptamer-MIP hybrid receptor for highly sensitive electrochemical detection of prostate specific antigen. Biosensors and Bioelectronics 75 , pp. 188-195. 10.1016/j.bios.2015.08.043

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Abstract

This study reports the design and evaluation of a new synthetic receptor sensor based on the amalgamation of biomolecular recognition elements and molecular imprinting to overcome some of the challenges faced by conventional protein imprinting. A thiolated DNA aptamer with established affinity for prostate specific antigen (PSA) was complexed with PSA prior to being immobilised on the surface of a gold electrode. Controlled electropolymerisation of dopamine around the complex served to both entrap the complex, holding the aptamer in, or near to, it’s binding conformation, and to localise the PSA binding sites at the sensor surface. Following removal of PSA, it was proposed that the molecularly imprinted polymer (MIP) cavity would act synergistically with the embedded aptamer to form a hybrid receptor (apta-MIP), displaying recognition properties superior to that of aptamer alone. Electrochemical impedance spectroscopy (EIS) was used to evaluate subsequent rebinding of PSA to the apta-MIP surface. The apta-MIP sensor showed high sensitivity with a linear response from 100 pg/ml to 100 ng/ml of PSA and a limit of detection of 1 pg/ml, which was three-fold higher than aptamer alone sensor for PSA. Furthermore, the sensor demonstrated low cross-reactivity with a homologous protein (human Kallikrein 2) and low response to human serum albumin (HSA), suggesting possible resilience to the non-specific binding of serum proteins.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RS Pharmacy and materia medica
Additional Information: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
Publisher: Elsevier
ISSN: 0956-5663
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 20 August 2015
Last Modified: 09 Sep 2020 11:15
URI: http://orca-mwe.cf.ac.uk/id/eprint/76261

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