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Inhibition of TGF-β1/Smad signal pathway is involved in the effect of Cordyceps sinensis against renal fibrosis in 5/6 nephrectomy rats

Pan, M., Zhang, M., Ni, H., Chen, J., Xu, M., Phillips, Aled Owen and Liu, B. 2013. Inhibition of TGF-β1/Smad signal pathway is involved in the effect of Cordyceps sinensis against renal fibrosis in 5/6 nephrectomy rats. Food and Chemical Toxicology 58 , pp. 487-494. 10.1016/j.fct.2013.04.037

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Abstract

The present study aimed to investigate the effects of Cordyceps sinensis on renal fibrosis and its possible mechanisms. Sprague-Dawley rats were randomly divided into three groups: sham operation (SHAM) group, 5/6 subtotal nephrectomy (SNx) untreated group, and 5/6 subtotal nephrectomy treated with C. sinensis (2.0. g/kg. d) (CS) group. Rats were studied 12. weeks after the surgery, and the CS group presented with significantly lower proteinuria, and better renal function compared with the SNx group (. p<. 0.05). Pathological study showed that the glomerulosclerosis tubulointerstitial injury score was significantly reduced in the CS group compared with the SNx group. Furthermore, the mRNA expression of TGF-β1, Smad2 and Smad3 and the protein expression of TGF-β1, TβRI, TβRII and p-Smad2/3 were attenuated by the C. sinensis treatment. In constrast, the mRNA and protein expression of Smad7 was upregulated. Furthermore, the expression of α-SMA and FSP1 was also significantly attenuated, accompanied by the increasing expression of E-cadherin, suggesting the inhibition of the epithelial-mesenchymal transition (EMT). In conclusion: C. sinensis exerted its antifibrotic effect on the SNx rats through the inhibition of the TGF-β1/Smad pathway.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
R Medicine > RZ Other systems of medicine
Uncontrolled Keywords: Animals;Base Sequence;Cordyceps;DNA Primers;Fibrosis;Kidney Diseases;Male;Nephrectomy;Plant Extracts;Rats;Rats Sprague-Dawley;Real-Time Polymerase Chain Reaction;RNA Messenger;Signal Transduction;Smad Proteins;Transforming Growth Factor beta1
Additional Information: EMTREE drug terms: alpha smooth muscle actin; beta actin; calvasculin; herbaceous agent; messenger RNA; Smad protein; Smad2 protein; Smad3 protein; Smad7 protein; transforming growth factor beta receptor 1; transforming growth factor beta receptor 2; transforming growth factor beta1; uvomorulin EMTREE medical terms: animal cell; animal experiment; animal model; animal tissue; article; beta actin gene; controlled study; drug mechanism; epithelial mesenchymal transition; experimental rat; gene expression; glomerulosclerosis; in vivo study; inhibition kinetics; kidney fibrosis; kidney function; male; nephrectomy; nonhuman; Ophiocordyceps; protein expression; rat; scoring system; signal transduction; Smad2 gene; Smad3 gene; SMAD7 gene; transforming growth factor beta1 gene; upregulation Medline keywords: Cordyceps sinensis; Renal fibrosis; TGF-β1 Medline is the source for the MeSH terms of this document.
Publisher: Elsevier
ISSN: 0278-6915
Last Modified: 04 Jun 2017 08:14
URI: http://orca-mwe.cf.ac.uk/id/eprint/74599

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