An in vitro test of the efficacy of an anti-biofilm wound dressing

Said, Jawal, Walker, Michael, Parsons, David, Stapleton, Paul, Beezer, Anthony E. and Gaisford, Simon 2014. An in vitro test of the efficacy of an anti-biofilm wound dressing. International Journal of Pharmaceutics 474 (1-2) , pp. 177-181. 10.1016/j.ijpharm.2014.08.034

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Abstract

Broad-spectrum antimicrobial agents, such as silver, are increasingly being formulated into medicated wound dressings in order to control colonization of wounds by opportunistic pathogens. Medicated wound dressings have been shown in-vitro to be effective against planktonic cultures, but in-vivo bacteria are likely to be present in biofilms, which makes their control and eradication more challenging. Recently, a functional wound dressing (AQUACEL® Ag+ Extra™ (AAg + E)) has been developed that in addition to silver contains two agents (ethylenediaminetetraacetic acid (EDTA) and benzethonium chloride (BC)) designed to disrupt biofilms. Here, the efficacy of AAg + E is demonstrated using a biofilm model developed in an isothermal microcalorimeter. The biofilm was seen to remain viable in the presence of unmedicated dressing, silver-containing dressing or silver nitrate solution. In the presence of AAg + E, however, the biofilm was eradicated. Control experiments showed that neither EDTA nor BC alone had a bactericidal effect, which means it is the synergistic action of EDTA and BC disrupting the biofilm with silver being bactericidal that leads to the product’s efficacy.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Staphylococcus aureus; Biofilm; Isothermal calorimetry; Wound dressings; silver
Additional Information:	Available online 21 August 2014
Publisher:	Elsevier
ISSN:	0378-5173
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	19 August 2014
Last Modified:	07 Nov 2023 07:25
URI:	https://orca.cardiff.ac.uk/id/eprint/73544

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