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U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor α

Jiang, Wen Guo, Puntis, M.C.A. and Hallett, Maurice Bartlett 1992. U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor α. Journal of Immunological Methods 152 (2) , pp. 201-207. 10.1016/0022-1759(92)90141-F

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Abstract

The U937 cell line has been shown to generate tumour fibrosis factor α (TNF-α) in response to soluble stimuli such as PMA and LPS, but only after treatment with GM-CSF. We report here the generation of TNF-α from U937 cells following phagocytosis of opsonised zymozan particles without the need for pre-treatment with GM CSF. The release of TNF-α from U937 cells was demonstrated by a specific radioimmunoassay, L929 cell killing and neutrophil ‘priming’. The biological activities in the cell supernatant were inhibited by TNF-α affarserum. Phagocytosis was required for TNF-α production. Non-opsonised zymozan or latex particles which were not phagocytosed or pretreatment with cytochalasin B, which inhibited phagocytosis of opsonised zymozan particles, all failed to trigger TNF-α production. Phagocytosis failed to trigger detectable IL-1 generation, and production of IL-6 was insufficient to produce biological effects on neutrophils. The U937 supernatant thus provides a source of human TNF-α which can be generated conveniently and cheaply for experimental investigations.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0022-1759
Last Modified: 11 Sep 2017 09:37
URI: http://orca-mwe.cf.ac.uk/id/eprint/72999

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