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The timing and magnitude of Ca2+ signaling by CD32 depends on its redistribution on the cell surface

Roberts, G., Davies, E. V., Pettit, E. J. and Hallett, Maurice Bartlett 1997. The timing and magnitude of Ca2+ signaling by CD32 depends on its redistribution on the cell surface. Experimental Cell Research 230 (2) , pp. 303-309. 10.1006/excr.1996.3416

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Abstract

Ca2+signaling was correlated with microaggregation and capping of CD32 molecules on the myeloid cell line, U937. The cytosolic free Ca2+signal was related to the extent of CD32 cross-linking and arose asymmetrically within individual cells. Both the magnitude and the delay before Ca2+signaling via CD32 on U937 cells was dependent on the extent of CD32 cross-linking. The delay time was extended in cells in which lateral diffusion in the membrane was reduced by covalently cross-linking of surface proteins. Under these conditions, capping but not surface microaggregation of CD32 molecules was prevented. The delay time before Ca2+signaling but not the magnitude was also affected. At a higher density of covalent cross-linking of surface proteins, the magnitude of the Ca2+signal by CD32 was also reduced and could be completely inhibited. This evidence therefore shows that the formation of a CD32 “cap” was not required for Ca2+signaling by this route. However, the signaling delay time was a consequence of lateral diffusion of CD32 molecules in the membrane to form signaling-competent microaggregates, and the redistribution of CD32 molecules on the cell surface was required for Ca2+signal generation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0014-4827
Last Modified: 04 Jun 2017 08:06
URI: http://orca-mwe.cf.ac.uk/id/eprint/72932

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