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Movement of strontium ions into mast cells and its relationship to the secretory response

Foreman, J. C., Hallett, Maurice Bartlett and Mongar, J. L. 1977. Movement of strontium ions into mast cells and its relationship to the secretory response. The Journal of Physiology 271 (1) , pp. 233-251. 10.1113/jphysiol.1977.sp011998

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Abstract

1. Non-stimulated mast cells take up (89)Sr and (45)Ca. There is a rapid phase of uptake of both labels which is complete in 1 min and the (89)Sr uptake is similar in magnitude to the (45)Ca uptake. A further slower phase of uptake occurs in the period from 1 to 30 min of incubation. The magnitude of this slower phase is about 6 times greater for (89)Sr than for (45)Ca.2. Non-stimulated and antigen-stimulated mast cells accumulate Sr which can be measured by atomic absorption spectroscopy. There is a direct relationship between Sr accumulation and histamine secretion which is independent of whether or not the cells are stimulated. 10% histamine secretion is associated with Sr accumulation of 0.25 f-mole/cell.3. The time course of (89)Sr uptake is similar to the time course of histamine secretion in non-stimulated cells.4. The uptake of (89)Sr is linearly related to the external Sr concentration in the range 0.5-16 m-mole/l. for both stimulated and non-stimulated cells.5. Ca, 10-1000 mumole/l. inhibits (89)Sr uptake in non-stimulated cells.6. Inhibition of glycolysis and oxidative phosphorylation prevents spontaneous histamine secretion in the presence of Sr without blocking the accumulation of Sr by the cells.7. Dibutyryl cyclic AMP, 10 m-mole/l. produces only 18% inhibition of (89)Sr uptake whereas histamine secretion is inhibited by 45% by the same concentration. The antigen-stimulated Sr uptake on the other hand can be completely inhibited by dibutyryl cyclic AMP, 10 m-mole/l.8. The uptakes of (89)Sr and Sr by unstimulated mast cells after 60 min incubation are dependent on extracellular H ion concentration. Both uptakes increase with increasing pH over the range pH 7-8.5.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
R Medicine > RZ Other systems of medicine
Uncontrolled Keywords: Animals,Bucladesine/pharmacology,Calcium/metabolism,Calcium/pharmacology,CellMembranePermeability,Female,HistamineRelease*/drugeffects,HydrogenIonConcentration,InVitroTechniques,Male,MastCells/drugeffects,Mast,Cells/metabolism*,Rats,SpectrophotometryAtomic,StimulationChemical,Strontium/metabolism*,Strontium/pharmacology,Time Factors Substances Bucladesine,Calcium,Strontium
Additional Information: Full Text Sources John Wiley & Sons, Inc. PubMed Central Other Literature Sources Access more work from the authors - ResearchGate Cited by Patents in - The Lens Molecular Biology Databases CALCIUM, ELEMENTAL - HSDB STRONTIUM, ELEMENTAL - HSDB Miscellaneous NCI CPTC Antibody Characterization Program
Publisher: The Physiological Society
ISSN: 0022-3751
Last Modified: 04 Jun 2017 08:05
URI: http://orca-mwe.cf.ac.uk/id/eprint/72728

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