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The use of single image random dot stereograms for presenting 3D microscopic confocal images

Hallett, Maurice Bartlett and Petit, E. J. 1997. The use of single image random dot stereograms for presenting 3D microscopic confocal images. Journal of Microscopy 186 (3) , pp. 275-278. 10.1046/j.1365-2818.1997.2130769.x

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Abstract

Presenting 3D images produced by confocal optical slicing techniques in a static and easily publishable form can be difficult. Here, we demonstrate the presentation of the data as a single image random dot stereogram (SIRD), which can be viewed as a 3D object by ‘defocusing’ the eyes. The production of the SIRD employs three steps: (i) acquisition of the optical slices using confocal techniques, (ii) allocation of a suitable grey level to the object in each slice to provide depth-encoding information for the final image and (iii) calculation of the SIRD from the composite depth-encoded image. The technique is demonstrated with a limited number of optical slices through an acridine-orange-stained neutrophil (diameter =10 μm), in order to show the relative positions of the nuclear lobes in the cell.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
R Medicine > RZ Other systems of medicine
Uncontrolled Keywords: Confocal microscopy,stereogram
Publisher: Wiley
ISSN: 0022-2720
Last Modified: 04 Jun 2017 08:05
URI: http://orca-mwe.cf.ac.uk/id/eprint/72671

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