Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

The regulation of copper stress response genes in the polychaete Nereis diversicolorduring prolonged extreme copper contamination

McQuillan, Jonathan S., Kille, Peter, Powell, Kate and Galloway, Tamara S. 2014. The regulation of copper stress response genes in the polychaete Nereis diversicolorduring prolonged extreme copper contamination. Environmental Science & Technology 48 (22) , pp. 13085-13092. 10.1021/es503622x

Full text not available from this repository.

Abstract

Polychaetes are frequented in toxicological studies, one reason being that some members occupy shallow burrows in sediments and are maximally exposed to the contaminants that accumulate within them. We have been studying one population of the polychaete Nereis (Hediste) diversicolor exhibiting inheritable tolerance to extreme copper contamination in estuarine sediment. Using transcriptome sequencing data we have identified a suite of genes with putative roles in metal detoxification and tolerance, and measured their regulation. Copper tolerant individuals display significantly different gene expression profiles compared to animals from a nearby population living without remarkable copper levels. Gene transcripts encoding principle copper homeostasis proteins including membrane copper ion transporters, copper ion chaperones and putative metallothionein-like proteins were significantly more abundant in tolerant animals occupying contaminated sediment. In contrast, those encoding antioxidants and cellular repair pathways were unchanged. Nontolerant animals living in contaminated sediment showed no difference in copper homeostasis-related gene expression but did have significantly elevated levels of mRNAs encoding Glutathione Peroxidase enzymes. This study represents the first use of functional genomics to investigate the copper tolerance trait in this species and provides insight into the mechanism used by these individuals to survive and flourish in conditions which are lethal to their conspecifics.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Publisher: American Chemical Society
ISSN: 0013-936X
Date of Acceptance: 22 October 2014
Last Modified: 21 Feb 2019 12:15
URI: http://orca-mwe.cf.ac.uk/id/eprint/72130

Citation Data

Cited 5 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item