Maintenance of in vivo induced cytochrome P-450s in hepatocyte monolayers at non freezing temperatures

Evans, Peter J. 2015. Maintenance of in vivo induced cytochrome P-450s in hepatocyte monolayers at non freezing temperatures. Cryobiology 70 (2) , pp. 204-207. 10.1016/j.cryobiol.2015.01.010

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Abstract

Cytochrome P450s (CYPs) induced in rats by 3-methylcholanthrene (3-MC), phenobarbital (PB) and dexamethasone (Dex) were investigated. The inducers had no effect on hepatocyte yield, viability, attachment or spreading on collagen. 3-MC induced ethoxyresorufin deethylase (EROD). Under normothermic conditions the activity fell in culture. However, it was maintained when cells were preserved at 10 °C under a gelatin gel. Upon reactivation the activity mirrored that of freshly isolated cells at 37 °C. Induced levels were stable for at least 6 h, the time to form a confluent monolayer. The investigation was extended to other CYPs by looking at patterns of testosterone metabolism. Phenobarbital had the greatest influence in terms of the quantity and number of metabolites. Culture at 37 °C decreased the peaks dramatically within 24 h. All 7 peaks were maintained in the preservation system.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Uncontrolled Keywords:	CYPs; Preinduced hepatocytes; Hypothermic preservation; Gelatin
Publisher:	Elsevier
ISSN:	0011-2240
Date of Acceptance:	29 January 2015
Last Modified:	28 Jun 2019 02:01
URI:	https://orca.cardiff.ac.uk/id/eprint/71538

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