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The phosphoinositide 3-kinase signaling pathway is involved in the control of modified low-density lipoprotein uptake by human macrophages

Michael, Daryn R., Davies, Thomas, Laubertová, Lucia, Gallagher, Hayley and Ramji, Dipak Purshottam 2015. The phosphoinositide 3-kinase signaling pathway is involved in the control of modified low-density lipoprotein uptake by human macrophages. Lipids 50 (3) , pp. 253-260. 10.1007/s11745-015-3993-0

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Abstract

The transformation of macrophages into lipid-loaded foam cells is a critical early event in the pathogenesis of atherosclerosis. Both receptor-mediated uptake of modified LDL, mediated primarily by scavenger receptors-A (SR-A) and CD36 along with other proteins such as lipoprotein lipase (LPL), and macropinocytosis contribute to macrophage foam cell formation. The signaling pathways that are involved in the control of foam cell formation are not fully understood. In this study, we have investigated the role of phosphoinositide 3-kinase (PI3K) in relation to foam cell formation in human macrophages. The pan PI3K inhibitor LY294002 attenuated the uptake of modified LDL and macropinocytosis, as measured by Lucifer Yellow uptake, by human macrophages. In addition, the expression of SR-A, CD36 and LPL was attenuated by LY294002. The use of isoform-selective PI3K inhibitors showed that PI3K-β, -γ and -δ were all required for the expression of SR-A and CD36 whereas only PI3K-γ was necessary in the case of LPL. These studies reveal a pivotal role of PI3K in the control of macrophage foam cell formation and provide further evidence for their potential as therapeutic target against atherosclerosis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Springer
ISSN: 0024-4201
Funders: British Heart Foundation
Date of First Compliant Deposit: 8 March 2019
Last Modified: 29 Jun 2019 16:01
URI: http://orca-mwe.cf.ac.uk/id/eprint/71004

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