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Crystal structure of fervidolysin from fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin

Kim, Jeong-Sun, Kluskens, Leon D., de Vos, Willem M., Huber, Robert and van der Oost, John 2004. Crystal structure of fervidolysin from fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin. Journal of Molecular Biology 335 (3) , pp. 787-797. 10.1016/j.jmb.2003.11.006

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Abstract

Structure-forming fibrous proteins like keratins, gelatins and collagens are degraded only by a few proteases as their tight packing limits access to the potential cleavage sites. To understand the keratin degradation in detail, we describe the first crystal structure of a keratin-degrading enzyme (keratinase), fervidolysin, from Fervidobacterium pennivorans as an immature form with propeptide (PD)-bound. The 1.7 Å resolution crystal structure shows that the protease is composed of four domains: a catalytic domain (CD), two β-sandwich domains (SDs), and the PD domain. A structural alignment shows a distant relationship between the PD–CD substructure of fervidolysin and pro-subtilisin E. Tight binding of PD to the remaining part of the protease is mediated by hydrogen bonds along the domain surfaces and around the active cleft, and by the clamps to SD1 and SD2. The crystal structure of this multi-domain protein fervidolysin provides insights into proenzyme activation and the role of non-catalytic domains, suggesting a functional relationship to the fibronectin (FN)-like domains of the human promatrix metalloprotease-2 (proMMP-2) that degrades the fibrous polymeric substrate gelatin.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0022-2836
Last Modified: 24 Jun 2017 11:00
URI: http://orca-mwe.cf.ac.uk/id/eprint/70171

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