Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Crystal structures of the tricorn interacting factor F3 from thermoplasma acidophilum, a zinc aminopeptidase in three different conformations

Kyrieleis, Otto J.P., Goettig, Peter, Kiefersauer, Reiner, Huber, Robert and Brandstetter, Hans 2005. Crystal structures of the tricorn interacting factor F3 from thermoplasma acidophilum, a zinc aminopeptidase in three different conformations. Journal of Molecular Biology 349 (4) , pp. 787-800. 10.1016/j.jmb.2005.03.070

Full text not available from this repository.

Abstract

The tricorn interacting factor F3 is an 89 kDa zinc aminopeptidase from the archaeon Thermoplasma acidophilum. Together with the tricorn interacting factors F1 and F2, F3 degrades the tricorn protease products and thus completes the proteasomal degradation pathway by generating free amino acids. Here, we present the crystal structures of F3 in three different conformations at 2.3 Å resolution. The zinc aminopeptidase is composed of four domains: an N-terminal saddle-like β-structure domain; a thermolysin-like catalytic domain; a small barrel-like β-structure domain; and an α-helical C-terminal domain, the latter forming a deep cavity at the active site. Three crystal forms provide snapshots of the molecular dynamics of F3 where the C-terminal domain can adapt to form an open, an intermediate and a nearly closed cavity, respectively. With the conserved Zn2+-binding motifs HEXXH and NEXFA as well as the N-terminal substrate-anchoring glutamate residues, F3 together with the leukotriene A4 hydrolase, represents a novel gluzincin subfamily of aminoproteases. We discuss the functional implications of these structures with respect to the underlying catalytic mechanism, substrate recognition and processing, and possible component interactions.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: zinc aminopeptidase; gluzincin; tricorn protease; proteasome; supermolecular complexes
Publisher: Elsevier
ISSN: 0022-2836
Last Modified: 24 Jun 2017 11:00
URI: http://orca-mwe.cf.ac.uk/id/eprint/70008

Citation Data

Cited 62 times in Google Scholar. View in Google Scholar

Cited 64 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item