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Crystal structure of an archaeal pentameric Rriboflavin synthase in complex with a substrate analog inhibitor: STEREOCHEMICAL IMPLICATIONS

Ramsperger, A., Augustin, M., Schott, A.-K., Gerhardt, S., Krojer, T., Eisenreich, W., Illarionov, B., Cushman, M., Bacher, A., Huber, Robert and Fischer, M. 2005. Crystal structure of an archaeal pentameric Rriboflavin synthase in complex with a substrate analog inhibitor: STEREOCHEMICAL IMPLICATIONS. Journal of Biological Chemistry 281 (2) , pp. 1224-1232. 10.1074/jbc.M509440200

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Abstract

Whereas eubacterial and eukaryotic riboflavin synthases form homotrimers, archaeal riboflavin synthases from Methanocaldococcus jannaschii and Methanothermobacter thermoautrophicus are homopentamers with sequence similarity to the 6,7-dimethyl-8-ribityllumazine synthase catalyzing the penultimate step in riboflavin biosynthesis. Recently it could be shown that the complex dismutation reaction catalyzed by the pentameric M. jannaschii riboflavin synthase generates riboflavin with the same regiochemistry as observed for trimeric riboflavin synthases. Here we present crystal structures of the pentameric riboflavin synthase from M. jannaschii and its complex with the substrate analog inhibitor, 6,7-dioxo-8-ribityllumazine. The complex structure shows five active sites located between adjacent monomers of the pentamer. Each active site can accommodate two substrate analog molecules in anti-parallel orientation. The topology of the two bound ligands at the active site is well in line with the known stereochemistry of a pentacyclic adduct of 6,7-dimethyl-8-ribityllumazine that has been shown to serve as a kinetically competent intermediate. The pentacyclic intermediates of trimeric and pentameric riboflavin synthases are diastereomers.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: American Society for Biochemistry and Molecular Biology
ISSN: 0021-9258
Last Modified: 24 Jun 2017 11:00
URI: http://orca-mwe.cf.ac.uk/id/eprint/69998

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