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Pulmonary oedema measured by MRI correlates with late-phase response to allergen challenge

Evans, Rhys L., Changani, Kumar K., Hotee, Sarah, Pindoria, Kashmira, Campbell, Simon, Nials, Anthony T., Ford, William Richard, Broadley, Kenneth John and Kidd, Emma Jane 2015. Pulmonary oedema measured by MRI correlates with late-phase response to allergen challenge. Experimental Lung Research 41 (4) , pp. 189-198. 10.3109/01902148.2014.985407
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Abstract

Purpose: Asthma is associated with reversible airway obstruction, leucocyte infiltration, airways hyperresponsiveness (AHR) and airways remodelling. Fluid accumulation causes pulmonary oedema contributing to airways obstruction. We examined the temporal relationship between the late asthmatic response (LAR) following allergen challenge of sensitised guinea-pigs and pulmonary oedema measured by magnetic resonance imaging (MRI). Materials and Methods: Ovalbumin (OVA) sensitised guinea-pigs received either a single OVA inhalation (acute) or nine OVA inhalations at 48 h intervals (chronic). Airways obstruction was measured as specific airways conductance (sGaw) by whole body plethysmography. AHR to inhaled histamine and bronchoalveolar lavage for leucocyte counts were measured 24 h after a single or the final chronic ovalbumin challenges. MRI was performed at intervals after OVA challenge and high intensity oedemic signals quantified. Results: Ovalbumin caused early bronchoconstriction, followed at 7 h by a LAR and at 24 h AHR and leucocyte influx. The bright intensity MRI oedema signal, peaking at 7 h, was significantly (P<0.05) greater after chronic (9.0±0.7x103 mm3) than acute OVA (7.6±0.2x103 mm3). Dexamethasone treatment before acute OVA abolished the AHR and LAR and significantly reduced eosinophils and the bright intensity MRI oedema from 9.1±1.0 to 6.4±0.3x103 mm3. Conclusion: We show a temporal relationship between oedema and the LAR and their parallel reduction, along with eosinophils and AHR, by dexamethasone. This suggests a close causative association between pulmonary oedema and impaired airways function.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RM Therapeutics. Pharmacology
Additional Information: Full text embargoed until 12 months after online publication. See http://www.sherpa.ac.uk/romeo/issn/0190-2148/ [accessed 21/11/14]
Publisher: Informa Healthcare
ISSN: 0190-2148
Funders: BBSRC -CASE studentship in collaboration with GlaxoSmithKline
Last Modified: 29 Jun 2019 15:59
URI: http://orca-mwe.cf.ac.uk/id/eprint/67568

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