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Identification and localization of concanavalin A binding sites on isolated postsynaptic densities

Gurd, J. W., Gordon-Weeks, P. R. and Evans, William Howard 1983. Identification and localization of concanavalin A binding sites on isolated postsynaptic densities. Brain Research 276 (1) , pp. 141-146. 10.1016/0006-8993(83)90555-3

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Abstract

Synaptic fractions of decreasing morphological complexity were prepared by phase partitioning of synaptic membranes in an aqueous two-phase polymer system containing increasing concentrations of the neutral detergent n-octylglucoside (OG). The morphology, distribution of concanavalin A binding sites (CABS) and protein and glycoprotein composition of the resultant fractions were examined. The lowest concentration of OG employed (0.5% w/w) gave fractions enriched in relatively intact junctions retaining both pre- and postsynaptic structures. Increasing the detergent concentration resulted in the stepwise solubilization of pre- and postsynaptic structures until purified postsynaptic densities (PSDs) were obtained with 1% (w/w) OG. CABS were generally distributed on all membrane structures present in the 0.5% OG fraction, were restricted to synaptic structures in the fraction obtained with 0.75% OG, and were localized to the convex (outer) surface of purified PSDs. Gel electrophoretic analysis showed that the restriction of CABS to the region of the synapse was associated with a marked increase in the concentration of glycoproteins with apparent molecular weights of 180,000 and 130,000. These glycoproteins were retained, and further concentrated in the purified PSD fraction.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
R Medicine > RZ Other systems of medicine
Publisher: Elsevier
ISSN: 0006-8993
Last Modified: 04 Jun 2017 07:28
URI: http://orca-mwe.cf.ac.uk/id/eprint/66756

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