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Process for producing active Wnt proteins in prokaryotes

Destree Olivier, Fahnert Beatrix, Neubauer Peter, Vainio Seppo, Veijola Johanna 2005. Process for producing active Wnt proteins in prokaryotes. WO2005123765 A1. [Patent].

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Abstract

This invention provides a process for producing active Wnt proteins in a bacterial host. The process comprises that a nucleic acid sequence encoding a Wnt protein is expressed in a bacterial host in an oxidative environment in the cytoplasm of a redox mutant strain or the expression product is directed out from the cytoplasm to an oxidative environment capable of retaining the activity of the Wnt protein. The Wnt protein product is recovered from the oxidative environment by recovery methods retaining the activity of the Wnt protein. This invention provides also active Wnt protein produced by the process.

Item Type: Patent
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QR Microbiology
Related URLs:
Last Modified: 12 Jun 2019 02:25
URI: http://orca-mwe.cf.ac.uk/id/eprint/65735

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