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Inclusion bodies: formation and utilisation

Fahnert, Beatrix, Lilie, Hauke and Neubauer, Peter 2004. Inclusion bodies: formation and utilisation. In: Enfors, S. -O. and Egli, Thomas eds. Physiological Stress Responses in Bioprocesses, Vol. 89. Advances in Biochemical Engineering, vol. 89. Berlin, Germany: Berlin/Heidelberg: Springer Verlag, pp. 93-142. (10.1007/b93995)

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Abstract

The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level production in bacterial hosts and simple optimisation protocols have not been available yet. Therefore, inclusion body (IB) based processes play a major role as a potential strategy for the production of complex recombinant proteins. These processes combine the advantages of a high accumulation of the target protein in well-characterised bacteria such as Escherichia coli, efficient strategies for IB isolation, purification and in vitro protein refolding without the need of complicated coexpression systems. Recent advances in the molecular physiology of IB formation and resolubilisation allow straight-forward optimisation of fermentation processes to obtain a high-quality product. In addition, simple strategies have been developed to optimise the purification and renaturation of disulfide bond containing proteins making a fast transfer of such processes into the industrial production scale realistic.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Bs; Aggregation; Proteolysis; Refolding; Stress response.
Additional Information: Series ISSN: 0724-6145.
Publisher: Berlin/Heidelberg: Springer Verlag
ISBN: 9783540203117
Last Modified: 12 Jun 2019 02:25
URI: http://orca-mwe.cf.ac.uk/id/eprint/65709

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