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An analysis of the genes encoding the 51.4- and 41.9-kDa toxins of Bacillus sphaericus2297 by deletion mutagenesis: the construction of fusion proteins

Oei, Coreen, Hindley, John and Berry, Colin 1990. An analysis of the genes encoding the 51.4- and 41.9-kDa toxins of Bacillus sphaericus2297 by deletion mutagenesis: the construction of fusion proteins. FEMS Microbiology Letters 72 (3) , pp. 265-273. 10.1111/j.1574-6968.1990.tb03900.x

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Abstract

A series of deletion mutants have been constructed, in which varying numbers of amino acids have been deleted from both the N- and C-termini of both the 51.4- and 41.9-kDa toxins of Bacillus sphaericus. The results show that between 34–39 and 52–54 amino acids respectively at the N- and C-termini of the 51.4-kDa protein, are not essential for toxicity. In the case of the 41.9-kDa protein, the removal of only 7 amino acids from the C-terminus abolishes toxicity whilst at least 17 amino acids can be deleted from the N-terminus without loss of toxicity. A fusion protein with the 51.4-kDa derived sequence N-terminal to the 41.9-kDa sequence yielded a protein of Mr 87 kDa which was not toxic by itself. When supplemented with cells expressing only the 51.4-kDa protein, toxicity was restored. In contrast, another fusion protein, in which the gene order was reversed, was shown to be fully active in toxicity assays.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: Bacillus sphaericus; Mosquitocidal toxins; Deletion mutants; Fusion proteins
Publisher: Wiley-Blackwell
ISSN: 0378-1097
Last Modified: 04 Jun 2017 06:47
URI: http://orca-mwe.cf.ac.uk/id/eprint/64836

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