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Changes in scleral collagen organization in murine chronic experimental glaucoma

Pijanka, Jacek K., Cone-Kimball, E., Pease, M. E., Abass, Ahmed, Sorensen, T., Nguyen, T. D., Quigley, H. A. and Boote, Craig 2014. Changes in scleral collagen organization in murine chronic experimental glaucoma. Investigative Ophthalmology and Visual Science 55 (10) , pp. 6554-6564. 10.1167/iovs.14-15047

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Purpose: The organization of scleral collagen helps to determine the eye's biomechanical response to intraocular pressure (IOP), and may therefore be important in glaucoma. This study provided a quantitative assessment of changes in scleral collagen fibril organization in bead-induced murine experimental glaucoma. Methods: Wide-angle x-ray scattering was used to study the effect of bead-induced glaucoma on posterior scleral collagen organization in one eye of 12 CD1 mice, with untreated fellow eyes serving as controls. Three collagen parameters were measured: (i) the local preferred fibril directions, (ii) the degree of collagen anisotropy and (iii) the total fibrillar collagen content. Results: The mouse sclera featured a largely circumferential orientation of fibrillar collagen with respect to the optic nerve head canal. Localized alteration to fibril orientations was evident in the inferior peripapillary sclera of bead-treated eyes. Collagen anisotropy was significantly (p < 0.05) reduced in bead-treated eyes in the superior peripapillary (Treated: 43 ± 8%; Control: 49 ± 6%) and mid-posterior (Treated: 39 ± 4%; Control: 43 ± 4%) sclera, and in the peripapillary region overall (Treated: 43 ± 6%; Control: 47 ± 3%). No significant differences in total collagen content were found between groups. Conclusions: Spatial changes in collagen fibril anisotropy occur in the posterior sclera of mice with bead-induced chronic IOP elevation and axonal damage. These results support the idea that dynamic changes in scleral form and structure play a role in the development of experimental glaucoma in mice, and potentially in human glaucoma.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Publisher: Association for Research in Vision and Ophthalmology
ISSN: 0146-0404
Last Modified: 17 Jun 2019 02:45

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