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Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation

Furuta, K. M., Yadav, S. R., Lehesranta, S., Belevich, I., Miyashima, S., Heo, J.-o., Vaten, A., Lindgren, O., De Rybel, B., Van Isterdael, G., Somervuo, P., Lichtenberger, R., Rocha, R., Thitamadee, S., Tahtiharju, S., Auvinen, P., Beeckman, T., Jokitalo, E. and Helariutta, Yrjo 2014. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation. Science 345 (6199) , pp. 933-937. 10.1126/science.1253736

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Photoassimilates such as sugars are transported through phloem sieve element cells in plants. Adapted for effective transport, sieve elements develop as enucleated living cells. We used electron microscope imaging and three-dimensional reconstruction to follow sieve element morphogenesis in Arabidopsis. We show that sieve element differentiation involves enucleation, in which the nuclear contents are released and degraded in the cytoplasm at the same time as other organelles are rearranged and the cytosol is degraded. These cellular reorganizations are orchestrated by the genetically redundant NAC domain–containing transcription factors, NAC45 and NAC86 (NAC45/86). Among the NAC45/86 targets, we identified a family of genes required for enucleation that encode proteins with nuclease domains. Thus, sieve elements differentiate through a specialized autolysis mechanism.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: American Association for the Advancement of Science
ISSN: 0036-8075
Last Modified: 19 Feb 2019 16:21

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