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CD59a deficient mice display reduced B cell activity and antibody production in response to T-dependent antigens

Sivasankar, Baalasubramanian, Donev, Rossen Mintchev, Longhi, M. Paula, Hughes, Timothy Richard, Davies, Ruth, Cole, Duncan Sean, Morgan, Bryan Paul and Marchbank, Kevin J. 2007. CD59a deficient mice display reduced B cell activity and antibody production in response to T-dependent antigens. Molecular Immunology 44 (11) , pp. 2978-2987. 10.1016/j.molimm.2006.12.025

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Abstract

CD59a is the primary regulator of membrane attack complex in mice. Recently, we have shown that CD59a-deficient (Cd59a−/−) mice exhibit enhanced CD4+ T cell responses. Here, we explored the effects of CD59a on B cell function and antibody production. Contrary to our expectations, Cd59a−/− mice showed a decreased humoral immune response to a T cell dependent antigen, sheep red blood cells. We found that the decreased humoral immune response was associated with a reduction in plasma cell number in vivo and reduced ability to respond to stimuli during in vitro culture experiments. Using MLR studies in which purified wild type or Cd59a−/− CD4+ T cells were mixed with purified B cells from each source, we found that the reduced B cell activation was largely due to the absence of CD59a on CD4+ T cells. Furthermore, a CD59a fusion protein bound specifically to mouse B cells, and enhanced B cell proliferation in a MLR, demonstrating that B cells express an as yet unidentified ligand for CD59a that aids in B cell activation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: National Institute of Health
ISSN: 0161-5890
Last Modified: 17 Apr 2019 21:03
URI: http://orca-mwe.cf.ac.uk/id/eprint/63640

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