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Voltage- and Ca2+-dependent burst generation in neuroendocrine dahlgren cells in the teleost platichthys flesus

Brierley, M. J., Bauer, C. S., Lu, W., Riccardi, Daniela, Balment, R. J. and McCrohan, C. R. 2004. Voltage- and Ca2+-dependent burst generation in neuroendocrine dahlgren cells in the teleost platichthys flesus. Journal of Neuroendocrinology 16 (10) , pp. 832-841. 10.1111/j.1365-2826.2004.01238.x

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The neuroendocrine Type 1 Dahlgren cells of the caudal neurosecretory system of the flounder display characteristic bursting activity, which may increase secretion efficiency. The firing activity pattern in these cells was voltage-dependent; when progressively depolarized, cells moved from silent (approximately −70 mV), through bursting and phasic to tonic firing (< −65 mV). Brief (10 s) evoked bursts of spikes were followed by a slow after-depolarization (ADP; amplitude up to 10 mV, duration 10–200 s), which was also voltage-dependent and could trigger a prolonged burst. The ADP was significantly reduced in the absence of external Ca2+ ions or the presence of the L-type Ca2+ channel blocker, nifedipine. BayK 8644 (which increases L-type channel open times) significantly increased ADP duration, whereas the Ca2+-activated nonselective cation channel blocker, flufenamic acid, had no effect. Pharmacological blockade of Ca2+-activated K+ channels, using apamin and charybdotoxin, increased the duration of both ADP and evoked bursts. However, action potential waveform was unaffected by either apamin/charybdotoxin, nifedipine, BayK 8644 or removal of external Ca2+. The short duration (approximately 100 ms), hyperpolarization-activated, postspike depolarizing afterpotentials (DAP), were significantly reduced by nifedipine. We propose that long duration ADPs underlie bursts and that short duration DAPs play a role in modulation of spike frequency.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Wiley-Blackwell
ISSN: 0953-8194
Last Modified: 04 Jun 2017 06:40

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