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Fatty acid modulation and sequence identity of fetal guinea pig alveolar type II cell amiloride-sensitive Na+ channel

Baines, D. L., MacGregor, G. G. and Kemp, Paul J. 2001. Fatty acid modulation and sequence identity of fetal guinea pig alveolar type II cell amiloride-sensitive Na+ channel. Biochemical and Biophysical Research Communications 288 (3) , pp. 727-735. 10.1006/bbrc.2001.5828

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Abstract

Removal of fetal lung fluid at birth is crucial to survival. In vivo, a reversal in the direction of vectorial, amiloride-sensitive Na+ transport can be stimulated by ETYA, a nonmetabolizable analogue of the naturally occurring unsaturated fatty acid, arachidonate. Using the patch-clamp technique, fetal guinea pig alveolar type II pneumocyte single Na+ channel activity was robustly activated by 10 μM arachidonate, ETYA, oleate and stearate; this was unaffected by cyclooxygenase and 5′lipoxygenase inhibitors. The Na+ channel expressed in fetal guinea pig alveolar epithelial type II pneumocytes has biophysical properties compatible with species-specific coexpression of a novel variant of αENaC with βENaC. γENaC is either not expressed in this tissue or shares very little homology with the rat and human γ subunit. Thus, dramatic stimulation of this channel by arachidonate explains the in vivo observation of gestation-dependent reversal of fetal transepithelial driving force and may, therefore, be of physiological significance during the transition to breathing air at birth.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0006-291X
Last Modified: 04 Jun 2017 06:39
URI: http://orca-mwe.cf.ac.uk/id/eprint/63421

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