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The effect of replication errors on the mismatch analysis of PCR-amplified DNA

Reiss, Jochen, Krawczak, Michael, Schloesser, Manfred, Wagner, Michael and Cooper, David Neil 1990. The effect of replication errors on the mismatch analysis of PCR-amplified DNA. Nucleic Acids Research 18 (4) , pp. 973-978. 10.1093/nar/18.4.973

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Abstract

The mismatch analysis of PCR-ampllfied DNA has generally assumed the absence of artificially introduced base substitutions in a significant proportion of the amplification product. This technique, however, differs from the direct sequencing of amplified DNA in that non-specific substitutions will render a molecule useless in analysis. The expected signal-to-noise ratio is heavily influenced by several parameters viz, initial template copy number, number of replication cycles, eventual product yield and the type of experimental system adopted. Mathematical modelling can be used to optimize fragment length with respect to the method applied and suggests as yet undescribed improvements such as partial modification or cleavage to optimize signal detection.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
ISSN: 0305-1048
Last Modified: 04 Jun 2017 06:33
URI: http://orca-mwe.cf.ac.uk/id/eprint/62059

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