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DNA detection rates of host mtDNA in bloodmeals of human body lice (Pediculus humanus L., 1758)

Davey, Jeffrey Stewart, Casey, C. S., Burgess, I. F. and Cable, Joanne 2007. DNA detection rates of host mtDNA in bloodmeals of human body lice (Pediculus humanus L., 1758). Medical and Veterinary Entomology 21 (3) , pp. 293-296. 10.1111/j.1365-2915.2007.00688.x

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Using polymerase chain reaction, we investigated the extent to which digestion affects the potential to amplify 12S mitochondrial DNA sequences from bloodmeals of individual human body lice (Pediculus humanus L.) (Phthiraptera, Pediculidae) up to 72 h after feeding on a surrogate rabbit host (Oryctolagus cuniculus L.) (Lagomorpha, Leporidae). Two rabbit-specific primer pairs were developed to produce amplicons of 199 bp and 283 bp, the smaller of which was found to have a significantly slower decay rate. Median detection periods (T50) for the amplicons were 20 h and 12 h, with maximum detection periods of 24 h and 12 h, respectively, suggesting an inversely proportional linear relationship between amplicon size and digestion time. The data provide an indication of timeframes essential for the design of forensic sampling protocols and a basis for investigating the feeding frequency of human lice.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QL Zoology
Uncontrolled Keywords: Pediculus humanus; digestion; DNA decay rates; forensic epidemiology; haematophagous; 12S mtDNA
Publisher: Wiley-Blackwell
ISSN: 0269-283X
Last Modified: 04 Jun 2017 06:33

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